Five human coronavirus nucleic acid detection kit (multiplex fluorescent PCR method)|BNCC378128 |BNCC

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Five human coronavirus nucleic acid testing kits (Multiple fluorescence PCR method)

Subculture procedure	Experimental preparation: dissolve the various components in the kit on ice before use, mix slightly and centrifuge for a short time, and place them on ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 23 μL, template 2 μL; Experimental reaction conditions: the first step is 96 ℃ for 10min, the second step is 95 ℃ for 10s, the third step is 53 ℃ for 40s, and the second to third steps are cycled 40 times;
Storage conditions	-20 ℃; protected from light
Safety level	1
application	Multiplex fluorescent PCR for rapid detection of HCoV-229E, HCoV-OC43, HKU1, NL63, SARS-CoV-2 five human coronavirus nucleic acids
Sharing mode	Public welfare sharing