Streptococcus agalactiae quantitative strains|BNCC340014 |BNCC

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Quantitative strains of streptococcus agalactiae

Culture medium	Special peptone: 23.0, soluble starch: 1.0, sodium chloride: 5.0, agar: 10.0, defibrated sheep blood: 5% (added at 50 ℃),pH:7.3±0.2(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	37 ℃;18-24h;5% CO2
Storage conditions	-20 ℃
Safety level	2
morphology	The colony is 1-2mm in diameter, round, with neat edges, opaque, white front, light color, convex in the middle, smooth surface, bright surface, moist texture, easy to stir up, no pigment, G + (blue-purple), cocci, purity: pure
Sharing mode	Public welfare sharing