BeNa Culture Collection

Human diffuse large B- cell lymphoma cells-BNCC
Human diffuse large B- cell lymphoma cells-BNCC
  • Human diffuse large B- cell lymphoma cells-BNCC
  • Human diffuse large B- cell lymphoma cells-BNCC
  • Human diffuse large B- cell lymphoma cells-BNCC
【OCI-LY3】

Human diffuse large B- cell lymphoma cells

  • Price: $273
  • number:BNCC338435
  • Form:
    Lymphoblast-like, round, multiple clusters, black spots in cell secretion
Basic package DNA extraction
Package:
  • Package A:STR report + 2 vials of frozen cell
  • Package B:STR report + 2 vials of frozen cell + 100ml of complete medium
Essential Information Certificate Related Products
Human diffuse large B- cell lymphoma cells
Culture medium RPMI-1640 complete medium: 80% RPMI-1640 + 20% FBS
Subculture procedure Recovery steps: ① Take out the frozen vial from liquid nitrogen or -80 ℃ refrigerator and put it into PE gloves, quickly submerse into a 37 ℃ water bath, shake the frozen vial to accelerate dissolution, and it is advisable to dissolve all within 1min; (2) Add the dissolved cell solution into a centrifuge tube containing 9ml of complete culture medium in an ultra-clean table, centrifuge at 1000-1200rpm for 3-5min, discard the supernatant, resuspend the cells with 1-2ml of complete media. ③The cell suspension was then added to the T25 flask containing 6-7mL of complete medium and placed in an incubator for culture. The culture flask is recommended to be cultured uprightly. cell subculture: ① centrifugation: collect cells, centrifuge at 1000rpm for 5min, discard the supernatant, resuspend the cells with 1-2ml of complete medium, add 1-2mL of culture solution and blow well, and dispense the cell suspension into a fresh T25 flask containing 8mL of culture medium according to a ratio of 1:2. (2) Half-volume solution exchange method: half-volume solution exchange method can be selected; Please gently suck half of the supernatant culture medium, resuspend and mix the remaining culture medium with cell precipitation, and dispense the cell suspension into a fresh T25 flask containing 8mL culture medium according to a ratio of 1:2. (3) Pay attention to the change of pH value of media and cell density, renew the media regularly (2-3 times a week), and repeat the subculture or cryopreservation when the cell density reaches more than 2 × 106 cells/ml.
Growth conditions Culture temperature 37 ℃; Atmosphere 5% CO2 + 95% air;
Growth characteristics Suspension growth
Storage conditions Liquid nitrogen
Type B cell lymphoma
Safety level 1
morphology Lymphoblast-like, round, multiple clusters, black spots in cell secretion
Separation substrate bone marrow
Sharing mode Public welfare sharing

1. Name:OCI-LY3

2. No.: 338435 

3. Growth properties :  □ wall ■ suspension □ semi-suspension and semi-wall 

4. Growth conditions:

culture medium 90% IMDM
serum 10%  FBS
temperature 37 ℃
air condition 5% CO2,95% AIR
growth algebra P4-5
frozen storage conditions culture medium 50%, serum 40%, DMSO 10%

5. Composition:

composition specifications
a bottle of cells T25
cell culture and operation instructions 1 copy

Receiving notice:

1 Upon arrival, it is suggested to sit the cells in a incubator for about 4 hours, and then renew the media for recovery or subculture according to the cell density.

2 Collect the cells by centrifuge, re-suspend the collected cells with 10ml of complete media, transfer to fresh culture flask/vessel and cultivate overnight, and dispense into separate vials for subculture according to the cell density.

3 Clustered growth: agitate the culture flask to disperse the clustered cells and continue to recover or subculture.

Recovery and subculture procedure of cells ( under strict aseptic conditions )

1 The suspension cells is normally handled by changing half of the solution and sub-cultured in separate flasks, that is, transfer half of the suspension liquid to a fresh flask/vessel, add appropriate complete medium and culture it in the incubator; It can also be sub-cultured in separate flasks according to cell density.

2 Pay attention to the change of pH value of medium and cell density, renew the medium regularly, and repeat the subculture or cryopreservation when the cell density reaches 70%-80%.

Special attention: (if handle in public laboratory or first time to culture the cells, it is recommended to add penicillin streptomycin into the media)

1 Upon the receipt of the cells, renew the solution with fresh 10% FBS medium as soon as possible. It is not recommended to use the medium used for transportation in the original flask.

2 Please take photos and contact the technicians in time if the culture flask leaks upon the receipt of cells.

3 Any compliant on the cells, please take photos and contctat our technicians.

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