BeNa Culture Collection

Human colon adenocarcinoma cells-BNCC
Human colon adenocarcinoma cells-BNCC
  • Human colon adenocarcinoma cells-BNCC
  • Human colon adenocarcinoma cells-BNCC
  • Human colon adenocarcinoma cells-BNCC
【SW948[SW-948]】

Human colon adenocarcinoma cells

  • Price: $273
  • number:BNCC295321
  • Form:
    Epithelial cell-like, irregularly shaped, with irregular margin, monolayer adherent growth, clean background, no pigment production, no vacuoles
Basic package DNA extraction
Package:
  • Package A:STR report + 2 vials of frozen cell
  • Package B:STR report + 2 vials of frozen cell + 100ml of complete medium
Essential Information Certificate Related Products
Human colon adenocarcinoma cells
Culture medium L-15 complete medium: 90% L-15 + 10% FBS
Subculture procedure Recovery steps: ① Take out the frozen vial from liquid nitrogen or -80 ℃ and put it into PE gloves, quickly submerse into a 37 ℃ water bath pot, shake the frozen vial to accelerate dissolution, and it is advisable to dissolve it completely within 1 minute; (2) Add the dissolved cell liquid into a centrifuge tube containing 9ml of complete culture medium in an ultra-clean table, centrifuge at 1000-1200rpm/min for 3-5 minutes, discard the supernatant, resuspend the cells with 1-2ml of complete media minutes. ③The cell suspension was then added to the T25 flask containing 5-6mL of complete medium and placed in an incubator for culture. Cell subculturing: ① remove the medium, rinse twice with PBS, and add 1-2mL pancreatin (0.25% Trypsin + 0.02% EDTA); (2) Observe the digestion situation under the microscope. When the cell edge shrinks and the adherent is loose (a pasteur pipette can be used to suck out a little pancreatin and gently blow somewhere in the cell layer, the cell layer can be seen to detach with naked eyes, that is, digestion is completed, otherwise digestion is continued), directly suck out pancreatin add 5-6ml of complete medium, gently blow the cell layer off. ③ Dispense the cell suspension into a fresh T25 flask as the ratio of 1:2, add appropriate complete culture medium, mix the cell suspension evenly, and culture in an incubator. ④ Pay attention to the change of PH of medium and cell density, renew the medium regularly (2-3 times a week), and repeat subculture or cryopreservation when the cell density reaches 80%-90%.
Growth conditions Culture temperature 37 ℃; Atmosphere 100% air;
Growth characteristics Adherent growth
Storage conditions Liquid nitrogen
Type adherent cell
Safety level 1
morphology Epithelial cell-like, irregularly shaped, with irregular margin, monolayer adherent growth, clean background, no pigment production, no vacuoles
Separation substrate large intestine colon
Sharing mode Public welfare sharing

SW948 human colon adenocarcinoma cells

BNCC number : 295321

Growth characteristics : Adherent growth

Growth conditions : 37 ℃,100% air

Complete medium: 90% L-15 + 10% FBS

Cryopreservation conditions: 50% basal medium + 40% FBS + 10% DMSO

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the cells are well. For the frozen cells,  it shall be stored in the refrigerator at -80 ℃ upon arrival. If they are not used for a long time, they shall be transferred to liquid nitrogen for storage overnight.  Recovered cells in T25 culture flask, upon receipt, put the culture flask in the incubator for 2-3h, and then carry out handling procedure. During recovery, each vial shall be used up once and shall not be retained. After recovery, the cells can be passed on to the next generation and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of cells are not be available in case of loss of cell viability.

Recovery steps: 
(1)The frozen vial is taken out of liquid nitrogen or -80 ℃ refrigerator and put into PE gloves, quickly submersed into a 37 ℃ water bath, shaken the frozen vial to accelerate dissolution, and it is advisable to dissolve all within 1min; 
(2)Put the dissolved cell solution into a centrifuge tube containing 9ml of complete culture medium in an ultra-clean table, centrifuge at 1000-1200rpm for 5min, discard the supernatant, ?resuspend the cells with 1-2ml of complete media. 
(3)The cell suspension was added to T25 flask containing 5-6ml complete medium and cultured in an incubator. 
Subculture: 
(1)remove the medium, rinse twice with PBS , and add 1-2mL pancreatin (0.25% Trypsin + 0.02% EDTA);
(2)Observe the digestion situation under the microscope. When the cell edge shrinks and the adherent is loose (a pasteur pipette can be used to suck up some pancreatin and gently blow somewhere in the cell layer, and the cell layer can be seen to detach with naked eyes, I .e. digestion is completed, otherwise digestion is continued), directly suck out pancreatin, add 5-6mL of complete medium, gently blow the cell layer off. 
(3)dispense the cell suspension into a fresh T25 flask as the ratio of 1:2, add appropriate complete culture medium, make the cell suspension well distributed, and culture in an incubator.
(4)Pay attention to the change of pH value of media and cell density, renew the media regularly (2-3 times a week), and repeat the passage operation or cryopreservation when the cell density reaches 80%-90%.
Notes:
(1)The cells are density dependent. Initiate the subcultivation in T25 flask when the cell density reaches 80%; A subcultivation ratio of 1:2 is recommended. 
(2)If the cells in sealed culture bottle, put it into the incubator for cultivation after handling, with the cap unscrewed.

(3)Cell culture is often accompanied by debris floating, can appropriately increase the frequency of fluid exchange.
Recovery record:  According to the recovery instructions, the results of the cell recovery are reported as follows:

Item quality standard recovery record
viability: adherence is observed in 18 hours,  the cell adherence rate ≥ 80.0% in 168 hours adherence is observed in 18 hours,  the cell adherence rate ≥ 80.0% in 144hours
cell morphology: epithelial cell-like epithelial cell-like, irregular, block growth
attached figure:
Conclusion: good viability, and no abnormal cell morphology, qualified ;
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