Neisseria gonorrhoeae (Zopf) Trevisan|BNCC356118 |BNCC

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Neisseria gonorrhoeae (Zopf) Trevisan

Culture medium	Columbia blood plate (CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃,5% CO2,2-3 days
Storage conditions	2-8 ℃; ship in ice bag
morphology	Round, neat edges, opaque, gray-white on the front, dark, convex in the middle, smooth and bright surface, moist texture, easy to pick up, no pigment, G-(red), cocci, purity: pure
application	Media testing Quality control strain Quality control strain for BBL products Sexually Transmitted Disease Research
Sharing mode	Public welfare sharing

Genomic DNA

Product content: 5μg ;

Purity： (A260/A280):1.7-2.0

Product format: Freeze-dried powder

Storage conditions: 2~8 ℃

Validity period: 6 months

Biosafety level: 1

Purpose: Molecular research

Notes: If you find any abnormal conditions such as damage on the day of receipt, please contact customer service within 24 hours; after opening, it should be used up once and cannot be stored. If it is not opened temporarily, it can be stored at -20°C. Please operate in strict accordance with this instruction, otherwise it will cause abnormality, deactivation, etc., and no reissue service will be provided.

Steps:

1. Prepare sterile ddH₂O;

2. centrifuge the sample tube 12000r /min for 1min, and add the corresponding volume of sterile ddH₂O as required;

3. It can be used normally after a short period of centrifugation after oscillation;