Akkermansia muciniphila DNA lab quality control|BNCC372221 |BNCC

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Akkermansia muciniphila DNA lab quality control

Culture medium	Peptone (trypsin hydrolysis):15.0, yeast extract powder: 5.0, glucose: 5.0, sodium thioglycolate: 0.5,L-cystine: 0.5, sodium chloride: 2.5, resazurin: 0.001, agar: 0.75,pH: 7.1±0.2(25 ℃)
Subculture procedure	Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: This reference is an inactivated particle. It needs to be used after nucleic acid extraction. The extraction or purification reagent should be prepared by itself. Avoid repeated freezing and thawing before extraction.
Growth conditions	37 ℃;4-5 days; anaerobic;
Storage conditions	-80 ℃
Safety level	1
application	The quality control is developed by Henan engineering center of industrial microbial strain, which is a mucophilic Ackerman bacteria inactivated virus liquid, which can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. by digital PCR value.
Sharing mode	Public welfare sharing