Human enterovirus 71 virus RNA lab quality control|BNCC368339 |BNCC

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Human enterovirus 71 virus RNA lab quality control

Culture medium	EMEM complete medium (with 2% FBS):EMEM + 2% FBS
Subculture procedure	Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl.
Growth conditions	Culture temperature 37 ℃; Gas environment 5% CO2 + 95% air;
Storage conditions	-80 ℃
Safety level	2
application	The quality control was developed by Henan engineering center of industrial microbial strain, which is an inactivated suspension of human enterovirus 71, which can be used for method establishment, working standard assignment, method confirmation and evaluation by adding stabilizer and digital PCR value.
Sharing mode	Public welfare sharing