Bovine adenovirus 3 DNA lab quality control|BNCC366628 |BNCC

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Bovine adenovirus 3 DNA lab quality control

Culture medium	EMEM complete medium (with 2% FBS):EMEM + 2% FBS
Subculture procedure	Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. Minimum sampling volume of 200 μl
Growth conditions	Culture temperature 37 ℃; Culture time 3-4 days; Gas environment 5% CO2 + 95% air;
Storage conditions	-20 ℃
Safety level	2
Separation substrate	Conjunctiva from a healthy cow.
application	The quality control is developed by Henan engineering center of industrial microbial strain, which is bovine adenovirus 3 inactivated virus liquid, which can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. through digital PCR value.
Sharing mode	Public welfare sharing