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African swine fever virus (ASF) nucleic acid detection kit certificate
1.Product information
Sample name: African swine fever virus (ASF) nucleic acid detection kit (fluorescence PCR method)
Sample number: 361557
Sample batch: 220513
2.Product features
3.Instruction
Experiment preparation: Dissolve the various components in the kit on ice before use, mix gently and centrifuge briefly, then place on ice for later use;
Experimental reaction system (25 μL): 15.5 μL of qPCR mixed reaction solution, 2 μL of template, and sterile ddH2O to make up to 25 μL;
Experimental reaction conditions: the first step is 95℃ for 1min, the second step is 95℃ for 10s, the third step is 10℃ for 30s, and the second to third steps are cycled 40 times; Fluorescence channel: FAM.
Negative: no exponential growth curve, or a lower growth curve and a Ct value >35;
Positive: There is an obvious exponential growth curve, and the Ct value is ≤35.
5. Notes
1. Dissolve the various components in the kit on ice before use, mix thoroughly and centrifuge briefly and place on ice for later use;
2. In order to ensure the sensitivity of the detection, it is recommended that the qPCR mixed reaction solution be frozen and thawed no more than 3 times;
3. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination.
Henan Engineering Technology Research Center of Industrial Microbial Strain
website: www.bncc.org.cn tel: 400-6699-833