BeNa Culture Collection
Subculture procedure | Experimental preparation: the various components in the kit are used to dissolve on ice, slightly mixed and briefly centrifuged, and then placed on ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 15.5 μL, template 2 μL, sterile ddH2O to 25 μL; Experimental reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 57 ℃ for 30s, and the second to third steps are cycled 40 times; Fluorescence channel: FAM. |
Storage conditions | -20 ℃, kept away from light |
Safety level | 1 |
application | It is applied to the specific in vitro amplification of African swine fever virus DNA by fluorescent PCR technology, which cannot be used for clinical diagnosis of diseases. |
Sharing mode | Public welfare sharing |
African swine fever virus (ASF) nucleic acid detection kit certificate
1.Product information
Sample name: African swine fever virus (ASF) nucleic acid detection kit (fluorescence PCR method)
Sample number: 361557
Sample batch: 220513
2.Product features
Reagent composition | 1 tube (240 μL) of qPCR mixed reaction solution; 1 tube (50 μL) of positive quality control material; 1 tube (200 μL) of sterile ddH2O | ||
Packing specifications | 15 T/box | Sensitivity | 30copies/μL |
Field of application | Applicable fields: It is suitable for the in vitro amplification research and detection of African swine fever virus, but cannot be used for clinical diagnosis of diseases | ||
Save and transport | -20 ℃, valid for 1 year, dry ice transportation | ||
*Note: If you have any questions about this kit, please contact our center (BNCC) for help before use |
3.Instruction
Experiment preparation: Dissolve the various components in the kit on ice before use, mix gently and centrifuge briefly, then place on ice for later use;
Experimental reaction system (25 μL): 15.5 μL of qPCR mixed reaction solution, 2 μL of template, and sterile ddH2O to make up to 25 μL;
Experimental reaction conditions: the first step is 95℃ for 1min, the second step is 95℃ for 10s, the third step is 10℃ for 30s, and the second to third steps are cycled 40 times; Fluorescence channel: FAM.
Negative: no exponential growth curve, or a lower growth curve and a Ct value >35;
Positive: There is an obvious exponential growth curve, and the Ct value is ≤35.
5. Notes
1. Dissolve the various components in the kit on ice before use, mix thoroughly and centrifuge briefly and place on ice for later use;
2. In order to ensure the sensitivity of the detection, it is recommended that the qPCR mixed reaction solution be frozen and thawed no more than 3 times;
3. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination.
Henan Engineering Technology Research Center of Industrial Microbial Strain
website: www.bncc.org.cn tel: 400-6699-833