African swine fever virus (ASF) nucleic acid detection kit (fluorescent PCR method)|BNCC361557 |BNCC

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African swine fever virus nucleic acid detection kit (QPCR)

Subculture procedure	Experimental preparation: the various components in the kit are used to dissolve on ice, slightly mixed and briefly centrifuged, and then placed on ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 15.5 μL, template 2 μL, sterile ddH2O to 25 μL; Experimental reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 57 ℃ for 30s, and the second to third steps are cycled 40 times; Fluorescence channel: FAM.
Storage conditions	-20 ℃, kept away from light
Safety level	1
application	It is applied to the specific in vitro amplification of African swine fever virus DNA by fluorescent PCR technology, which cannot be used for clinical diagnosis of diseases.
Sharing mode	Public welfare sharing

African swine fever virus (ASF) nucleic acid detection kit certificate

1.Product information

Sample name: African swine fever virus (ASF) nucleic acid detection kit (fluorescence PCR method)

Sample number: 361557

Sample batch: 220513

2.Product features

Reagent composition	1 tube (240 μL) of qPCR mixed reaction solution; 1 tube (50 μL) of positive quality control material; 1 tube (200 μL) of sterile ddH2O
Packing specifications	15 T/box	Sensitivity	30copies/μL
Field of application	Applicable fields: It is suitable for the in vitro amplification research and detection of African swine fever virus, but cannot be used for clinical diagnosis of diseases
Save and transport	-20 ℃, valid for 1 year, dry ice transportation
*Note: If you have any questions about this kit, please contact our center (BNCC) for help before use

3.Instruction

Experiment preparation: Dissolve the various components in the kit on ice before use, mix gently and centrifuge briefly, then place on ice for later use;

Experimental reaction system (25 μL): 15.5 μL of qPCR mixed reaction solution, 2 μL of template, and sterile ddH2O to make up to 25 μL;

Experimental reaction conditions: the first step is 95℃ for 1min, the second step is 95℃ for 10s, the third step is 10℃ for 30s, and the second to third steps are cycled 40 times; Fluorescence channel: FAM.

Negative: no exponential growth curve, or a lower growth curve and a Ct value >35;

Positive: There is an obvious exponential growth curve, and the Ct value is ≤35.

5. Notes

1. Dissolve the various components in the kit on ice before use, mix thoroughly and centrifuge briefly and place on ice for later use;

2. In order to ensure the sensitivity of the detection, it is recommended that the qPCR mixed reaction solution be frozen and thawed no more than 3 times;

3. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833

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