BeNa Culture Collection
Subculture procedure | Experimental preparation: dissolve the various components in the kit on ice before use, mix slightly and centrifuge for a short time, and place them on ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 15.5 μL, template 2 μL, sterile ddH2O to 25 μL; Experimental reaction conditions: the first step 95 ℃ 1min, the second step 95 ℃ 10s, the third step 57 ℃ 30s, the second to third steps cycle 40 times; Fluorescence Channel: FAM. |
Storage conditions | -20 ℃, protected from light |
Safety level | 1 |
application | The specific in vitro amplification of African swine fever virus DNA by fluorescence PCR technology can not be used for clinical diagnosis of diseases. |
Sharing mode | Public welfare sharing |