Vibrio cholerae nucleic acid detection kit (fluorescent PCR method)|BNCC361344 |BNCC

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Vibrio cholerae nucleic acid detection kit (fluorescent PCR method)

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Sharing mode	Public welfare sharing

Vibrio cholerae nucleic acid test kit certificate

1.Product information

Sample name: Vibrio cholerae nucleic acid detection kit (fluorescent PCR method)

Sample number: 361344

Sample batch: 220420

2. Product features

Reagent composition	qPCR mixed reaction solution 1 tube (525μL); Positive quality control 1 tube (100μL); Negative quality control 1 tube (100μL)
packing specifications	25 T/box
sensitivity	1.64copies/μL
applicable fields	Suitable for auxiliary diagnosis of Vibrio cholerae infection in water-like feces, suspected contaminated water, food and other samples
save and transport	-20 ℃, valid for 1 year, dry ice transportation
*Note: If you have any questions about this kit, please contact our center (BNCC) for help before use

3. Use steps

1. Experimental preparation: Dissolve the various components in the kit on ice before use, mix gently and centrifuge briefly, and place on ice for later use;

2. Sample processing: sample processing according to "SN/T 5125-2019 Vibrio cholerae Inspection";

3. Reagent preparation: experimental reaction system (25 μL), 21 μL qPCR mixed reaction solution, 4 μL template;

4. Reaction conditions: 95℃ 3min 1cycle; 94℃ 10s, 48℃ 30s 40cycle;

5. Fluorescence channel: CY5;

4. Result judgment

1. The sample has a typical amplification curve, and the Ct value is less than or equal to 35;

2. The sample has no amplification curve, and the Ct value is ≥40 or not detected;

3. If the t value of the sample is >35 and <40, it needs to be re-measured. Retest results Ct value ≥ 40 is negative, Ct value < 40 is positive.

5. Preservation and transportation

1. Dissolve the various components in the kit on ice before use, mix thoroughly and centrifuge briefly and place on ice for later use;

2. In order to ensure the sensitivity of the detection, it is recommended that the qPCR mixed reaction solution be frozen and thawed no more than 3 times;

3. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination;

Henan Engineering Research Center of Industrial Microbiology

Website:www.bncc .org .cn tel: 400-6699-833