BeNa Culture Collection
Culture medium | Peptone: 10.0, beef extract powder: 8.0, yeast powder: 4.0, glucose: 20.0, magnesium sulfate: 0.2, sodium acetate: 5.0, diammonium citrate: 2.0, dipotassium hydrogen phosphate: 2.0, manganese sulfate: 0.04, tween 80:1.0, agar: 14.0,pH:5.7±0.2(25 ℃) |
Subculture procedure | Inoculate MRS agar plate culture medium, wash and resuspend with PBS, and inactivate in a water bath at 65 ℃ for 30min. |
Growth conditions | 37 ℃;24-48h; anaerobic; |
Storage conditions | -80 ℃ |
Safety level | 1 |
application | The quality control is developed by Henan engineering center of industrial microbial strain, which is the inactivated suspension of Jen's Lactobacillus, which can be used for method establishment, work standard assignment, method confirmation and evaluation, product quality control, etc. by adding stabilizer and digital PCR fixed value. |
Sharing mode | Public welfare sharing |