Porphyromonas gingivalis nucleic acid reference (Heat inactivated) (Strongly positive)|BNCC363616 |BNCC

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Porphyromonas gingivalis nucleic acid reference (Heat inactivated) (Strongly positive)

Culture medium	Columbia blood plate (CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	(1) Prepare the above two plates; (2) After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then crush it with tweezers; (3) Absorb 0.5mL of sterile water (placed in anaerobic environment for 24 hours) into a freeze-drying tube, fully dissolve the bacterial powder, then punch into 2 plates, 200 μL/piece, and coat evenly; ④ Place the plate under the above culture conditions, and the strain can be used when it grows out.
Growth conditions	37 ℃;8-10 days; Anaerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 1-2mm, round, with neat edges, opaque, black on the front, convex in the middle, smooth surface, bright surface, moist texture, easy to stir up, G-(red), coccolidia, purity: pure
Separation substrate	Human gingival sulcus
application	Quality control strain
Sharing mode	Public welfare sharing