Klebsiella pneumoniae nucleic acid reference (heat inactivated) (strong positive)|BNCC361497 |BNCC

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Klebsiella pneumoniae nucleic acid reference (heat inactivated) (strong positive)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	This reference does not recommend repeated freezing and thawing. Before use, take it out of the refrigerator, balance to room temperature, mix well, and use it.
Growth conditions	Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic gas environment;
Storage conditions	-80 ℃
Safety level	2
morphology	Colony morphology: colonies formed on DHL agar plate are light pink, large and raised, smooth and moist, mucus-like, and adjacent colonies are easy to melt into pus-like; Microscopic morphology: Gram-negative Brevibacterium, without spores, single, double or short-chain oval or rod-shaped.
application	This reference product is developed by Henan Industrial Microbial Strain Engineering Technology Research Center. It can be used for specific analysis of nucleic acid detection kits, cross-reaction verification, measurement method evaluation and laboratory quality control.
Sharing mode	Public welfare sharing

Klebsiella pneumoniae nucleic acid reference

This reference product was developed by Henan Engineering Technology Research Center of Industrial Microbial Strain and can be used for specific analysis of nucleic acid detection kits, cross-reaction verification, measurement method evaluation and laboratory quality control.

1. Product information

Product name	Klebsiella pneumoniae nucleic acid reference (heat inactivated) (strongly positive)
No.	BNCC 361497	batch	220512

2. Product features

Description	6 pieces of this product, each containing 0.5mL, frozen
Species	Bacterial kingdom, & gamma;-Proteobacteria, Enterobacteriaceae, Klebsiella, Klebsiella pneumoniae
Traceability	Henan industrial microbial strain engineering technology research center & larr;CMCC(B)46117
Preparation method	inoculation broth medium, centrifugal collection, PBS suspension, 80 ℃ water bath inactivation for 1 hour
Other ingredients	PBS, stabilizer.
Store	-80℃	Validity period	2 years
* Note: If you have any questions about reference items, please contact BNCC for help

3. Quality inspection

Item	standard	results (* see appendix for confirmation of test data)
Bacterial concentration	≥1.0×10⁷CFU/mL	5.0×10⁷CFU/mL
Authentication	phenotypic characteristics, biochemical identification, gene sequencing	completely consistent with Klebsiella pneumoniae, and the similarity rate is 100%
fluorescence quantification	PCR ≤25ct	(22.8±0.1)ct

4. Instructions

This reference product is not recommended for repeated freezing and thawing. Before use, take it out of the refrigerator, equilibrate to room temperature, mix well, and it is ready to use.

5. Notes

1. After receiving the goods, if any damage is found, please contact the center in time within 24 hours, and reissue it free of charge after confirmation;

2. This product uses heat inactivation method, which is likely to cause protein damage, and is only suitable for nucleic acid and other tests;

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833