BeNa Culture Collection
Culture medium | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
Subculture procedure | This reference does not recommend repeated freezing and thawing. Before use, take it out of the refrigerator, balance to room temperature, mix well, and use it. |
Growth conditions | Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic gas environment; |
Storage conditions | -80 ℃ |
Safety level | 2 |
morphology | Colony morphology: colonies formed on DHL agar plate are light pink, large and raised, smooth and moist, mucus-like, and adjacent colonies are easy to melt into pus-like; Microscopic morphology: Gram-negative Brevibacterium, without spores, single, double or short-chain oval or rod-shaped. |
application | This reference product is developed by Henan Industrial Microbial Strain Engineering Technology Research Center. It can be used for specific analysis of nucleic acid detection kits, cross-reaction verification, measurement method evaluation and laboratory quality control. |
Sharing mode | Public welfare sharing |
Klebsiella pneumoniae nucleic acid reference
This reference product was developed by Henan Engineering Technology Research Center of Industrial Microbial Strain and can be used for specific analysis of nucleic acid detection kits, cross-reaction verification, measurement method evaluation and laboratory quality control.
1. Product information
Product name | Klebsiella pneumoniae nucleic acid reference (heat inactivated) (strongly positive) | ||
No. | BNCC 361497 | batch | 220512 |
2. Product features
Description | 6 pieces of this product, each containing 0.5mL, frozen | ||
Species | Bacterial kingdom, & gamma;-Proteobacteria, Enterobacteriaceae, Klebsiella, Klebsiella pneumoniae | ||
Traceability | Henan industrial microbial strain engineering technology research center & larr;CMCC(B)46117 | ||
Preparation method | inoculation broth medium, centrifugal collection, PBS suspension, 80 ℃ water bath inactivation for 1 hour | ||
Other ingredients | PBS, stabilizer. | ||
Store | -80℃ | Validity period | 2 years |
* Note: If you have any questions about reference items, please contact BNCC for help |
3. Quality inspection
Item | standard | results (* see appendix for confirmation of test data) |
Bacterial concentration | ≥1.0×107CFU/mL | 5.0×107CFU/mL |
Authentication | phenotypic characteristics, biochemical identification, gene sequencing | completely consistent with Klebsiella pneumoniae, and the similarity rate is 100% |
fluorescence quantification | PCR ≤25ct | (22.8±0.1)ct |
4. Instructions
This reference product is not recommended for repeated freezing and thawing. Before use, take it out of the refrigerator, equilibrate to room temperature, mix well, and it is ready to use.
5. Notes
1. After receiving the goods, if any damage is found, please contact the center in time within 24 hours, and reissue it free of charge after confirmation;
2. This product uses heat inactivation method, which is likely to cause protein damage, and is only suitable for nucleic acid and other tests;
Henan Engineering Technology Research Center of Industrial Microbial Strain
website: www.bncc.org.cn tel: 400-6699-833