Fecal coliform standard quality control sample (multi-tube fermentation method)|BNCC359289 |BNCC

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Fecal coliform standard quality control sample (Multitube fermentation method)

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	Samples required a total of 100mL of sterile water or sterile saline to dissolve. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biosafety cabinet, 3~4mL of sterile water is immediately added, dissolved, sucked out and injected into a sterile bottle, the remaining sterile water is repeatedly washed with the inner wall of the vial, and all transferred to the above sterile bottle, which is the standard quality control sample, equivalent to 100mL of water sample to be tested, with a concentration of 300 ~ 3000MPN/L.
Growth conditions	Culture temperature 37 ℃; Culture time 24-48 hours; Aerobic gas environment
Storage conditions	-20 ℃, protected from light
Safety level	1
application	It is suitable for the determination of fecal coliform in water quality, quality control and personnel assessment (multi-tube fermentation method). Reference standard "HJ 347.2-2018 water quality-Determination of fecal coliforms-Multi-tube fermentation method"
Sharing mode	Public welfare sharing