Bacillus coagulans spore suspension|BNCC361969 |BNCC

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Bacillus coagulans spore suspension

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	1. Cultivate the target bacteria until the spore yield is maximum; 2. Inactivate and dissolve the bacteria themselves and collect spores; 3. Count and pack the spores;
Growth conditions	45 ℃;24-48h; Aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	This spore suspension is a pure spore suspension, which is obtained by cell separation and collection of spores. It can be used directly, and the corresponding volume can be added according to the test requirements.
application	It is suitable for the production of sterilization effect indicator bacteria and high-density Bacillus coagulans in canned food.
Sharing mode	Public welfare sharing