Bacillus coagulans spore suspension|BNCC362557 |BNCC

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Bacillus coagulans spore suspension

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	45 ℃;24-48; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony is 0.5-1mm in diameter, round, with neat edges, opaque, gray-white front, convex in the middle, bright surface, smooth surface, moist texture, easy to stir up, G + (blue-purple), bacilli
application	It is suitable for the production of sterilization effect indicator bacteria for canned food and high density Bacillus coagulans.
Sharing mode	Public welfare sharing