BeNa Culture Collection
Culture medium | Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
Subculture procedure | 1. According to the arrow sign of penicillin bottle cap, open the strain and dissolved liquid; 2. transfer the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve; 3. After full dissolution, it is 100 ~ 500CFU/ml, which is absorbed in proportion according to the requirements; 4. Please use it up immediately after opening and do not store it; |
Growth conditions | 37 ℃;18-24h; Aerobic; |
Storage conditions | -20 ℃ |
morphology | The colony diameter is 1-2mm, round, with neat edges, opaque, yellow on the front, light color, convex in the middle, bright surface, smooth surface, moist texture, easy to pick up, G-(red), bacilli |
Separation substrate | Sputum |
application | Type strain. Water quality testing, quality control strains. |
Sharing mode | Public welfare sharing |
Enterobacter aerogenes
Strain number:BNCC 358452 / CMCC(F)98001
Name: Enterobacter aerogenes
Product form: quantitative bacteria tablets Number of viable bacteria:100~500CFU/bottle
Product batch:22012501 Valid until:2023.01.24
Inspection date: 2022.01.26 Sampling ratio: ≥2%
Report number:RS22356329-1 Biosafety level: 1, handle in ultra-clear table or safety cabinet
Item | description | results |
stain microscopy | gram negative and positive, cell morphology observed under microscope | G-bacilli |
colony morphology | observation of single colony morphology | Light yellow, moist, raised, with neat edges |
Purity | non-selective medium culture, colony observation and microscopic examination | No contaminated bacterial |
Reference value of live bacteria | Non-selective medium plate, viable count | 240CFU/bottle |
Phenotypic and Genotypic Analysis | phenotypic testing, gene sequencing | qualified |
test conclusion | qualified |
Product list: 100 ~ 500CFU quantitative strain 1 bottle, 1mL dissolved solution 1 bottle, strain certificate 1 copy;
Validity period: unopened, below -20 ℃, 12 months;
Usage:
1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;
2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;
3. After fully dissolved, it is 100 ~ 500CFU/mL, which can be absorbed in proportion according to the test requirements;
4. Please use up immediately after opening, and do not store it;
Notes:
① This product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.
② Used bacteria-carrying consumables, please autoclave and discard them.