Quantitative strain of Escherichia coli|BNCC354967 |BNCC

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Quantitative strain of Escherichia coli

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	1. According to the arrow sign of penicillin bottle cap, open the strain and dissolved liquid; 2. transfer the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve; 3. After being fully dissolved, it can be absorbed proportionally according to the requirements; 4. Please use it up immediately after opening and do not store it;
Growth conditions	37 ℃;18-24h; Aerobic;
Growth characteristics	G-bacilli, lysine decarboxylase, ornithine decarboxylase positive, arginine dihydrolase negative, urease, phenylalanine deaminase negative, IMViC test: +, +,-,-;MUG positive, cellobisaccharide, side calendolol negative, glucose ammonium positive; no H2S, lactose fermentation acid production gas. Facultative anaerobic, optimum pH7.4 ~ 7.6.
Storage conditions	-20 ℃
morphology	Pale yellow, raised, moist, bright, G-bacilli
application	Research; Analytical testing. Bacteria for research and quality control, "GB 4789.2-2010 colony count determination", "GB 4789.3-2010 coliform count", research; Analysis and testing. "GB/T 4789.32-2002 Coliform Rapid Test" and "GB 4789.39-2013 Fecal Coliform Count" Positive Control Strain, Quality Control Strain of Chinese Pharmacopoeia, 2015 Edition.
Sharing mode	Public welfare sharing

Escherichia coli

Strain number:BNCC 354967 / CMCC 44102

Name: Escherichia coli

Product format: quantitative bacteria tablets Number of viable bacteria: (1.7±0.5)×10^4CFU/bottle

Product batch: 220322 Valid until: 2024.03.21

Inspection date: 2022.03.23 Sampling ratio: ≥2%

Report number: RS22354967-1 Biosafety level: 1, handle in ultra-clear table or safety cabinet

Item	Inspection description	results
stain microscopy	gram negative and positive, cell morphology observed under microscope	G-bacilli
colony morphology	observation of single colony morphology	pale yellow, raised, moist, bright
Purity	non-selective medium culture, colony observation and microscopic examination	No contaminated bacterial
Reference value of live bacteria	Non-selective medium plate, viable count	1.7×10^4CFU/bottle
Phenotypic and Genotypic Analysis	phenotypic testing, gene sequencing	qualified
test conclusion	After the BNCC quality control inspection system quality inspection, the product is qualified

product list: One bottle of (1.7±0.5)×10^4CFU quantitative strain, one bottle of 1mL dissolution solution and one copy of strain certificate;

shelf life: unopened, below -20 ℃, 24 months;

Usage:

1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;

2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;

3. after full dissolution, it is (1.7±0.5)×10^4CFU/mL, can be absorbed in proportion according to the test requirements;

4. Please use up immediately after opening, and do not store it;

Notes:

① This product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.

(2) Used bacteria-carrying consumables, please autoclave and discard them.