Alternaria apple|BNCC359824 |BNCC

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Alternaria apple

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, the colonies are obvious, brown, dense and vigorous on the integrated PDA medium, spreading and growing to the edge of the plate, and the back of the medium is light yellow.
Separation substrate	Plants/apples/leaves
Sharing mode	Public welfare sharing

Alternaria mali Roberts

Storage conditions : 2~8 ℃

No. :359824

Product format :agar slant in 14mm test tube

Validity :growing culture, in 30 days

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28 ℃, aerobic, integrated PDA,5-7 days. Comprehensive PDA: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2 Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;

(4) lay flat the small pieces to the center of the agar plate;

(5)put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability in 7days,strain layer is obvious
colony morphology:	Small filamentous fungi, with obvious colonies on comprehensive PDA medium, brown, Dense exuberant, spreading to the edge of the plate, light yellow on the back of the medium
Conclusion:	good viability, no abnormal colony morphology, qualified

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