Malassezia restricta|BNCC359455 |BNCC

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Malassezia restricta

Culture medium	Pityrosporum culture medium (MFM): wort agar 60.0g, bovine bile (dry) 20.0g, Tween 40 10.0g,Glycerol mono-oleate 2.5g, distilled water 1.0L. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) the frozen vial is dissolved in a water bath at 30 ℃, and the surface is sterilized and opened in the biosafety cabinet; (3) sucking bacteria liquid with a pasteur pippette, dispense on the plate evenly; (4) placing the plate under the above culture conditions for cultivation.
Growth conditions	30 ℃;5-7 days; Aerobic;
Storage conditions	-80 ℃
morphology	The colony diameter is 1-2mm, round, with neat edges, opaque, milky white, convex in the middle, smooth and bright surface, moist texture, easy to stir up, G + (blue-purple), cocci, purity: pure
Separation substrate	Human skin
application	Classification; Research
Sharing mode	Public welfare sharing

Malassezia restricta

Storage conditions : 2~8 ℃

No. : 359455

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 30 ℃, aerobic, pityrosporum culture medium: wort agar 60.0g, bovine bile (dry) 20.0g, twain 40 10.0g,Glycerol mono-oleate 2.5g, distilled water 1.0 L. Sterilization at 121 ℃ for 15min. 5-7 days. (Once the strain is transferred to culture, it cannot be placed below 30 ℃, and it needs to be transferred every 3-4 weeks.)

Recovery steps:

① Prepare the above flat plate 1-2 block;

②The frozen storage tube is dissolved in a 30 ℃ water bath, and the surface is disinfected and opened in a safety cabinet;

③ Use a pasteurized pipette to suck the bacteria liquid, punch it into the plate, and coat it evenly;

④ Place the plate under the above culture conditions for 5-7 days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability， in 7days strain layer is obvious;colony is typical on the streaked plate
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: light yellow uplift: middle raised surface: rough fold texture: dry
Conclusion	good viability, no abnormal colony morphology, qualified