NRS 1221A|BNCC358668 |BNCC

BeNa Culture Collection

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NRS 1221A-BNCC
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  • Bacillus subtilis-BNCC

Bacillus subtilis

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  • number：BNCC358668
Standard strain Quantitative strain DNA extraction
Package：
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
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Essential Information Related Products
Bacillus subtilis
Culture medium Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 30 ℃;18-24h; Aerobic;
Storage conditions 2-8 ℃
application Resistance Testing
Dry heat sterilization control
Efficacy testing
Ethylene oxide sterilization control
Media testing
Steam sterilization control
Sterility assurance
Sterilization control
Pharmaceutical and Personal Care Resistance test
dry heat sterilization control
efficacy test ethylene oxide sterilization control
medium inspection BR> sterility assurance
sterilization control> drugs and personal care
Sharing mode Public welfare sharing
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