BeNa Culture Collection

Malassezia slooffiae-BNCC
Malassezia slooffiae-BNCC
Malassezia slooffiae-BNCC
  • BNCC
  • Malassezia slooffiae-BNCC
  • Malassezia slooffiae-BNCC
  • Malassezia slooffiae-BNCC

Malassezia slooffiae

  • Price: $ 286
  • number:BNCC357899
  • Form:
    Colony diameter 1-2mm, round, irregular edge, opaque, gray-white front, convex middle, rough surface, easy to pick up, G (blue-purple), oval
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
Essential Information Certificate Related Products
Malassezia slooffiae
Culture medium Pityrosporum culture medium (MFM): wort agar 60.0g, bovine bile (dry) 20.0g, Tween 40 10.0g,Glycerol mono-oleate 2.5g, distilled water 1.0L. Sterilization at 121 ℃ for 15min.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 30°C ;24-48h; Aerobic;
Storage conditions 2-8 ℃
morphology Colony diameter 1-2mm, round, irregular edge, opaque, gray-white front, convex middle, rough surface, easy to pick up, G (blue-purple), oval
Sharing mode Public welfare sharing

Malassezia Slofi

Storage conditions : 2~8 ℃

No. : 357899

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of  agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 30 ℃, aerobic, chaff spore medium, 24-48h. Pityrospore medium: wort agar 60.0g, bovine bile (dry) 20.0g, Tween 40 10.0g,Glycerol mono-oleate 2.5g, distilled water 1.0L. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates;

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 24-48 hours. 

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

Item test results
viability good viability, in 48hours strain layer is obvious;colony is typical on the streaked plate

colony morphology

(above)

Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque

color: white bulge: middle bulge surface: rough texture: easy to stir

Conclusion good viability, no abnormal colony morphology, qualified

 

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