Malassezia sympodialis|BNCC357896 |BNCC

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Malassezia sympodialis

Culture medium	Pityrosporum culture medium (MFM): wort agar 60.0g, bovine bile (dry) 20.0g, Tween 40 10.0g,Glycerol mono-oleate 2.5g, distilled water 1.0L. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30°C;48-72h; Aerobic;
Storage conditions	2-8 ℃
morphology	The colony diameter is 1-2mm, round, with neat edges, opaque, convex in the middle, smooth and bright surface, moist texture, easy to stir up, golden yellow on the front, golden yellow on the back, G + (blue-purple), cocci, purity: pure
Sharing mode	Public welfare sharing

Malassezia sympodialis

Storage conditions : 2~8 ℃

No. : 357896

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:30 ℃, aerobic, chaff spore medium, 48-72h. Pityrospore medium: wort agar 60.0g, bovine bile (dry) 20.0g, Tween 40 10.0g,Glycerol mono-oleate 2.5g, distilled water 1.0L. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates;

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 48-72 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability， in 72hours strain layer is obvious;colony is typical on the streaked plate
colony morphology (above)	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque color: light yellow uplift: middle convex surface: bright and smooth texture: moist and easy to stir
Conclusion	good viability, no abnormal colony morphology, qualified