BeNa Culture Collection

Parabacteroides merdae (Johnson et al. 1986) Sakamoto and Benno 2006  Hahnke et al. 2016-BNCC
  • BNCC
  • Parabacteroides merdae (Johnson et al. 1986) Sakamoto and Benno 2006  Hahnke et al. 2016-BNCC

Parabacteroides merdae (Johnson et al. 1986) Sakamoto and Benno 2006 Hahnke et al. 2016

  • Price: $ 172
  • number:BNCC342254
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
Essential Information Related Products
Parabacteroides merdae (Johnson et al. 1986) Sakamoto and Benno 2006 Hahnke et al. 2016
Culture medium Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 37°C;48-72h; Anaerobic
Storage conditions 2-8 ℃
Sharing mode Public welfare sharing
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