Streptomyces microflavus|BNCC337579 |BNCC

Streptomyces microflavus

Price： $ 143
number：BNCC337579
Form：
G + size: 2-4mm shape: round edge: irregular transparency: opaque color: white uplift: middle convex surface: rough fold texture: firm hard and dry, neat edge, front white, light color, smooth surface, gray surface, dry texture, difficult to pick/cut, no pigment, G + (blue purple), bacilli, purity: pure

Standard strain Quantitative strain DNA extraction

Package：

freeze dried
Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
agar slant
Agar slant: growing culture, ready to use, Short term preservation, convenient access

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Streptomyces microflavus

Culture medium	Gauche Synthesis No. 1 Agar (GM): 20g of soluble starch, 0.5g of sodium chloride, 0.01g of ferrous sulfate, 1.0g of potassium nitrate, 0.5g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 15g of agar, 1.0L of distilled water and 7.3±0.2 of pH. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of the plate and open it in biosafety cabinet; (3) Absorb 1-3ml of sterile water into the plate, scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make a bacterial suspension; (4) suck the bacteria suspension with a pasteur pipette, dispense it into a fresh plate evenly. (5) put the plates under the growth conditions.
Growth conditions	28 ℃;3-5 days; Aerobic
Storage conditions	2-8 ℃
morphology	G + size: 2-4mm shape: round edge: irregular transparency: opaque color: white uplift: middle convex surface: rough fold texture: firm hard and dry, neat edge, front white, light color, smooth surface, gray surface, dry texture, difficult to pick/cut, no pigment, G + (blue purple), bacilli, purity: pure
Sharing mode	Public welfare sharing

1.Description

1. Name:Streptomyces microflavus

2. BNCC No.:337579

3. Biosafety level: 4

2. Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth conditions

1. Gao's synthetic agar no 1: KNO3 1g, soluble starch 20g ,K2HPO4 0.5g, MgSO4.7H2O 0.5g ,NaCl 0.5g ,FeSO4 0.01g, agar 20g, water 1000ml, pH 7.2-7.4

2. Atmosphere: aerobic

3. Temperature: 28 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization.