Streptococcus agalactiae|BNCC336970 |BNCC

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Streptococcus agalactiae

Culture medium	Tryptone soybean blood plate (TSA-B): tryptone 15.0g, soybean peptone 5.0g, sodium chloride 5.0g, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;18-24h; Aerobic
Growth characteristics	Lan's group B
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: white bulge: middle bulge surface: bright and smooth texture: moist and viscous
Sharing mode	Public welfare sharing

1.Description

1. Name: Streptococcus agalactiae

2. BNCC No.:336970

3. Biosafety level: 4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1, TSA + 5% defibrinated sheep blood: tryptone (casein trypsin digest) 15.0g, soybean peptone (soybean powder papain digest) 5.0g, sodium chloride 5.0g, agar 15.0g,5% defibrinated sheep blood, pH7.3±0.2. 121 ℃,15min.

2. Atmosphere:facultative anaerobic

3. Temperature: 37 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization.