BeNa Culture Collection
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Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
Agar slant: growing culture, ready to use, Short term preservation, convenient access
| Culture medium | Streptomyces medium No. 2 (ISP-2): yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH 7.2±0.2. Sterilization at 121 ℃ for 15min. |
| Subculture procedure | (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow. |
| Growth conditions | 28 ℃;3-5 days; Aerobic; |
| Storage conditions | 2-8 ℃ |
| morphology | Size: 1-2mm Shape: round Edge: Irregular Transparency: Opacity Color: light yellow Protuberance: Middle bulge Surface: Rough Folds Texture: dry and easy to stir up |
| application | Erythromycin production |
| Sharing mode | Public welfare sharing |
Streptomyces erythreus
Storage conditions : 2~8 ℃
No. 335903
Product format: freeze dried, 200ul
Validity : 6 years
Biosafety level : 1 , handle in ultra-clean table or safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions :28 ℃, aerobic, Streptomyces medium No. 2, 3-5d. Streptomyces medium No. 2: yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH 7.2±0.2. Sterilization at 121 ℃ for 15min.
Recovery steps:
(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;
(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
| Item | test results |
| viability: | good viability,in 3days strain layer become obvious, colony is typical |
| colony morphology: (above) |
Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opacity Color: light yellow uplift: middle raised surface: rough fold texture: dry and easy to stir |
| Conclusion: | good viability, no abnormal colony morphology, qualified |
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