Streptomyces sp.|BNCC335894 |BNCC

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Streptomyces sp.

Culture medium	Streptomyces medium No. 2 (ISP-2): yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH 7.2±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;3-5 days; Aerobic;
Growth characteristics	Gram-positive, after 14 days of growth in the medium, the hyphae are well developed, without transverse septum, not broken, the aerogenous hyphae are thinner, more branched, and curved, hooked or irregular spiral spore filaments are produced. The spores are oval, larger, and the surface is rough with small spines.
Storage conditions	2-8 ℃
morphology	Size: 2-4mm Shape: round Edge: serrated Transparency: Opacity Color: light yellow Protuberance: Middle bulge Surface: Rough Folds Texture: solid and hard
application	Classification; Research. Production of aureomycin (Aureomycin)
Sharing mode	Public welfare sharing

1.Description

1. Name: Streptomyces sp.

2. BNCC No.: 335894

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. Gao's synthetic agar No. 1: soluble starch 20.0g,KNO3 1.0 g,K2HPO4 0.5 g,MgSO4·7H2O 0.5 g,NaCl 0.5 g,FeSO4 0.01 g, agar 15.0g, distilled water 1.0 L,pH 7.2-7.4.

2. Atmosphere: aerobic

3, Temperature: 28-30 ℃

4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization