Aspergillus fumigatus Fresenius, anamorph|BNCC288647 |BNCC

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Aspergillus fumigatus Fresenius, anamorph

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of sterile water into a freeze dried ampoule, fully rehydrate, inoculate a plate with the solution at 200ul/plate; (4) Put the plate under the above culture conditions for 3-5 days.
Growth conditions	28 ℃,3-5 days, aerobic
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies on the integrated PDA medium, the plate layer mycelium is fluffy, dark green, and green spores are produced in the later period. The back of the medium is light yellow.
application	Research on Emerging Infectious Diseases Respiratory studies Study on opportunistic pathogens
Sharing mode	Public welfare sharing

Aspergillus fumigatus

Storage conditions: 2~8 ℃

No. : 288647

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level: 2 , handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :28 ℃, aerobic, integrated PDA,3-5 days. Comprehensive PDA: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 ·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 3-5 days.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability,in 4 days strain layer is obvious
colony morphology:	small filamentous fungi with obvious colonies on comprehensive PDA medium, the mycelium of the plate layer is fluffy, dark green, green spores are produced in the later stage, and the back of the medium is light yellow
Conclusion:	good viability, no abnormal colony morphology, qualified;