Trichoderma asperellum|BNCC227595 |BNCC

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Trichoderma asperellum

Culture medium	Potato Glucose Agar (PDA): Potato Boiled Solution 1.0L, Glucose 20.0g, Agar 15.0g, Natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Growth conditions	28 ℃,5-7 days, aerobic
Storage conditions	2-8 ℃
Separation substrate	Soil
Sharing mode	Public welfare sharing

1.Description

1. Name：Trichoderma asperellum

2. BNCC No.：227595

3. Biosafety level: 4

2. Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth conditions

1, PDA medium: potato extract 1.0 L, glucose 20.0g, agar 15.0g, natural pH.

2. Atmosphere：aerobic

3. Temperature：25-28 ℃

4.Notes：

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization