Bacillus coagulans|BNCC223353 |BNCC

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Bacillus coagulans

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O when culturing Bacillus. it is beneficial to produce spores.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	40 ℃,18-24h, aerobic
Growth characteristics	Colony round, flat, shiny, milky white edge, wavy, rod-shaped single arrangement contact enzyme, V-P determination, hydrolyzed gelatin, hydrolyzed starch-can grow in the range of 30 ℃-50 ℃, and can grow in a wide range of salt tolerance. Growth on beef paste medium containing 2-10% NaCl
Storage conditions	2-8 ℃
morphology	Size: 1-4mm shape: round edge: irregular transparency: opaque Color: light yellow uplift: flat surface: bright and smooth texture: moist and easy to stir up
application	Classification; Research; Teaching
Sharing mode	Public welfare sharing

1.Description

1. Name：Bacillus coagulans

2. BNCC No.：223353

3. Biosafety level：4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Medium components: peptone 5.0g, beef extract 3.0g,NaCl 5.0g, agar 15.0g, distilled water 1.0L,pH7.0. [Note] Add 5mg MnSO4·H2O when culturing Bacillus,which is beneficial to produce spores..

2. Atmosphere：aerobic

3. Temperature：40 ℃

4.Notes：

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization