Gibberella fujikuroi|BNCC187705 |BNCC

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Gibberella fujikuroi

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions	28 ℃;3-5 days; Aerobic;
Storage conditions	-196 ℃
morphology	Small filamentous fungi, with obvious colonies, white, dense and vigorous hyphae on the integrated PDA medium, spreading to the edge of the plate, and yellowish on the back of the medium.
Sharing mode	Public welfare sharing

Gibberella fujikuroi

Storage conditions: 2~8 ℃

No. 187705

Product format: agar slant in 14mm test tube

Validity: growing culture, in 30 days

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Gorwth conditions: 28 ℃, aerobic, integrated PDA,3-5 days.
Comprehensive PDA: Potato boiling liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, vitamin B1 trace amount, agar 20.0g, pH 6.0±0.2. Sterilize at 121℃ for 15min.

Potato boiling liquid : weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0l.

Receiving notice: If any abnormality is found on the day of receipt, please contact customer service in time within 24 hours. The tube bevel can be used directly. In principle, it can be used many times without stain during the storage period, but the vitality will gradually decrease with time. Please operate in strict accordance with the instructions. Otherwise, the replacement service will not be provided if abnormal strains and inactivation are caused.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;

(4) lay flat the small pieces to the center of the agar plate;

(5)put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 4days strain layer become obvious
colony morphology:	small filamentous fungi with obvious colonies on comprehensive PDA medium, white, hyphae are dense and vigorous, spreading to the edge of the plate, and the back of the medium is light yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified