Bifidobacterium bifidum|BNCC186493 |BNCC

Essential Information Certificate Related Products

Bifidobacterium bifidum

Culture medium	Bifid enrichment medium (BBL): peptone 15.0g, glucose 20.0g, yeast extract 2.0g, soluble starch 0.5g, sodium chloride 5.0g,L-cysteine 0.5g, tomato powder 5.0g, liver powder 2.0g, Tween 80 1mL, agar 20.0g (excluding liquid), distilled water 1.0L,pH value 7.0±0.1. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;24-48h; Anaerobic;
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: white bulge: middle bulge surface: bright and smooth texture: moist and sticky
Separation substrate	Adult intestinal tract
application	Used in the production of probiotic health food.
Sharing mode	Public welfare sharing

Bifidobacterium bifidum

Storage conditions: 2~8 ℃

No. 186493

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 37°C, anaerobic, BBL medium for 24-48h. BBL medium: peptone 15.0g, glucose 20.0g, yeast extract 2.0g, soluble starch 0.5g, sodium chloride 5.0g, L-cysteine 0.5g, tomato extract powder 5.0g, liver extract powder 2.0g, Warm 80 1mL, agar 20.0g (not included in liquid medium), distilled water 1.0L, pH 7.0±0.1. Sterilize at 121℃ for 15min.

Recovery steps:

① Prepare sterile test tube of 5-10ml liquid medium and 2 plates; Silica gel plug is used for liquid test tube of liquid medium to ensure ventilation and facilitate deoxygenation. Please put the liquid medium and plate in an anaerobic environment for 24h before usage.

② Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

③ Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

④ Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 24-48 hours strain layer become obvious;colony is typical on the streaked plate
colony morphology: (above)	size: 1-2mm ; Shape: round ; Edge: neat ; Transparency: opaque color: white ; Protuberance: middle bulge ; Surface: bright and smooth ; Texture: moist and viscous
Conclusion:	good viability, no abnormal colony morphology, qualified