Aspergillus nidulans|BNCC185653 |BNCC

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Aspergillus nidulans

Culture medium	Malt juice agar (MA): malt extract 20.0g, glucose 20.0g, peptone 1.0g, agar 20.0g, distilled water 1.0L,pH natural. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic;
Growth characteristics	The colony texture is fleece; the color of the colony is yellowish brown, and the old back edge becomes dark golden yellow; the reverse side is purple-red; the colony is flat, the entire edge, and the center point is convex; the exudate is a small amount of transparent small droplets at the beginning, and then becomes Larger lavender-red droplets. The head of the conidia is spherical or short columnar; the apical sac is hemispherical, and the surface is 1/3-2/3 fertile; the spore-producing structure is double-layered; the conidia are spherical and the wall is rough.
Storage conditions	2-8 ℃
morphology	Filamentous fungi have obvious colonies on the comprehensive PDA medium, dense and vigorous hyphae, spreading and growing, the initial hyphae are white, and the late hyphae turn yellow, with ring lines, and yellow spores.
application	Research.
Sharing mode	Public welfare sharing

Aspergillus nidulans

Storage conditions: 2~8 ℃

No,: 185653

Product format: freeze dried

Validity period: 24 months

Biosafety level: 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28-30 ℃, aerobic, integrated PDA. Comprehensive PDA: potato boiling juice 1000mL, potassium dihydrogen phosphate 3.0g, magnesium sulfate heptahydrate 1.5g, glucose 20.0g, vitamin B1 10mg, agar 20g,pH natural. 121 ℃,15min. Potato boiling solution: 200g of potato, peeled, cut into pieces, boiled in distilled water for 30min, constant volume of filtrate to 1000mL for later use. 121 ℃,15min.

Recovery steps:

(1)Prepare 1-2 pieces of SDA plates;

(2) Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, directly dispense 200ul of the liquid suspension into a SDA agar plate evenly.

(4) Put the plates under the above culture conditions for cultivation for 5-7 days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability,in 5 days plate colony is obvious
colony morphology:	The morphology of the bacteria is obviously visible in the integrated PDA agar medium, and the colony edge is white, yellow-green spores are produced, dry and vigorous, and spread to the entire flat surface.
conclusion:	good viability, no abnormal colony morphology, completely consistent with the above figure, qualified