Lactobacillus plantarum quantitative strain|BNCC364013 |BNCC

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Quantitative strains of lactobacillus plantarum

Culture medium	Peptone: 10.0, beef extract powder: 8.0, yeast powder: 4.0, glucose: 20.0, magnesium sulfate: 0.2, sodium acetate: 5.0, diammonium hydrogen citrate: 2.0, dipotassium hydrogen phosphate: 2.0, manganese sulfate: 0.04, Tween 80:1.0, agar: 14.0,pH:5.7±0.2(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	37 ℃;18-24h;5% CO2
Storage conditions	-80 ℃
Safety level	1
morphology	The colony is 1-2mm in diameter, round, with neat edges, Milky White front, opaque, convex in the middle, smooth surface, bright surface, moist texture, easy to pick up, G + (blue-purple), bacilli, purity: pure
Sharing mode	Public welfare sharing