Pseudomonas aeruginosa nucleic acid detection kit (fluorescence PCR method)|BNCC361453 |BNCC

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Pseudomonas aeruginosa nucleic acid detection kit (QPCR)

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	Experimental preparation: before the experiment, refer to T/SDAQI 005-2021 to carry out the enrichment culture of the sample; Dissolve the various components in the kit on ice with the front, mix slightly and centrifuge for a short time, and then place them on ice for standby; Reagent preparation: experimental reaction system (25μL),qPCR mixed reaction solution 18.5 μL, template 1 μL, sterile ddH2O5.5μL Reaction conditions: 95 ℃ 1min 1cycle;95 ℃ 10s, 50 ℃ 30s 40cycle; Fluorescence Channel: FAM.
Growth conditions	Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic atmosphere;
Storage conditions	-20 ℃, dark and refrigerated
Safety level	2
Separation substrate	Blood culture
application	For rapid detection of Pseudomonas aeruginosa in food and cosmetics.
Sharing mode	Public welfare sharing