BeNa Culture Collection
Subculture procedure | Sample treatment: sample treatment shall be carried out in accordance with GB 4789.8-2016 National Food Safety Standard-Examination of Yersinia enterocolitica; Experimental preparation: dissolve the various components in the kit on ice before use, mix slightly and centrifuge for a short time, and place them on ice for later use; Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 16 μL, template 1 μL, sterile ddH2O 8 μL Reaction conditions: 95 ℃ 1min 1cycle;95 ℃ 10s, 57 ℃ 30s 40cycle; Fluorescence channel: VIC; |
Safety level | 1 |
application | It is suitable for the auxiliary diagnosis of Yersinia enterocolitica infection in samples such as water stool, suspected contaminated water and food. |
Sharing mode | Public welfare sharing |