Listeria monocytogenes nucleic acid detection kit (fluorescent PCR method)|BNCC361444 |BNCC

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Listeria monocytogenes nucleic acid detection kit (QPCR)

Subculture procedure	Sample treatment: sample treatment shall be carried out in accordance with GB 4789.30-2016 National Food Safety Standard-Detection of Listeria monocytogenes; Experimental preparation: dissolve the various components in the kit on ice before use, mix slightly and centrifuge for a short time, and place them on ice for later use; Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 15 μL, template 1 μL, sterile ddH2O 9 μL Reaction conditions: 95 ℃ 1min 1cycle;95 ℃ 10s, 57 ℃ 30s 40cycle; Fluorescence channel: FAM;
Safety level	0
application	It is suitable for auxiliary diagnosis of Listeria monocytogenes infection in water samples, suspected contaminated water and food samples.
Sharing mode	Public welfare sharing