Proteus mirabilis nucleic acid detection kit (fluorescent PCR method)|BNCC370492 |BNCC

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Proteus mirabilis nucleic acid detection kit (Fluorescent PCR method)

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	Experimental preparation: refer to GB4789.10 or GB7918.5 to carry out the enrichment culture of the sample before the experiment; dissolve the various components in the kit on ice with the front, mix slightly and centrifuge for a short time, and then place them on ice for standby; Experimental reaction system (25 μL):qPCR mixed reaction solution 24 μL, template 1 μL; Experimental reaction conditions: the first step 95 ℃ 1min, the second step 95 ℃ 10s, the third step 51 ℃ 30s, the second to third steps cycle 40 times; Fluorescence channels: FAM, VIC. Experimental preparation: refer to GB4789.10 or GB7918.5 to carry out the enrichment culture of the sample before the experiment; dissolve the various components in the kit on ice with the front, mix slightly and centrifuge for a short time, and then place them on ice for standby; Experimental reaction system (25 μL):qPCR mixed reaction solution 24 μL, template 1 μL; Experimental reaction conditions: the first step 95 ℃ 1min, the second step 95 ℃ 10s, the third step 51 ℃ 30s, the second to third steps cycle 40 times; Fluorescence channels: FAM, VIC.
Growth conditions	30 ℃;18-24h; aerobic
Storage conditions	-20 ℃
Safety level	0
application	For rapid detection of Proteus mirabilis
Sharing mode	Public welfare sharing