Clostridium sporogenes quantitative strain|BNCC372992 |BNCC

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Quantitative strains of Clostridium sporogenes

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	30 ℃;18-24h; aerobic;
Storage conditions	-80 ℃
Safety level	1
morphology	The colony is 1-2mm in diameter, round, with irregular edges, opaque, gray-white front, light color, flat shape, rough surface, dark surface, dry texture, easy to provoke, G + (blue-purple), bacilli
application	research; analysis and detection. Teaching and scientific research. "Disinfection Technical Specification" Bacteria for Testing Sterilization Effect of Traditional Chinese Medicine Instruments, "GB/T 4789.12-2003 Clostridium Botulinum and Botulinum Toxin Test" Positive Control Strains, 2015 Edition of Chinese Pharmacopoeia Quality Control Strains. 2020 edition of pharmacopoeia-"general 1100 biological examination method" test strain pharmacopoeia bacteria, medium "sensitivity examination" test strain, "method applicability test" test strain, "medium applicability test and control bacteria test method applicability test" test strain
Sharing mode	Public welfare sharing