Campylobacter jejuni and Campylobacter coli nucleic acid detection kit (duplex fluorescent PCR method)|BNCC370236 |BNCC

Essential Information Related Products

Campylobacter jejuni and Campylobacter coli nucleic acid detection kit (Fluorescent PCR method)

Subculture procedure	1. Experimental preparation: dissolve the various components in the kit on ice before use, mix them slightly and centrifuge them briefly and place them on ice for later use; 2. Sample treatment: sample treatment shall be carried out according to SN/T 5125-2019 Inspection of Vibrio parahaemolyticus and Vibrio cholerae; 3. Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 21 μL, template 4 μL; 4. Reaction conditions: 95 ℃ 3min 1cycle;94 ℃ 10s, 50 ℃ 30s 40cycle; 5. Fluorescence channel: FAM (Vibrio parahaemolyticus), CY5 (Vibrio cholerae);
Storage conditions	-20 ℃
Safety level	0
application	It is suitable for auxiliary diagnosis of Campylobacter jejuni and Campylobacter coli infection in water samples, stool, suspected contaminated water, food and other samples.
Sharing mode	Public welfare sharing