Bacillus subtilis quantitative strain|BNCC361420 |BNCC

Essential Information Related Products

Quantitative strains of bacillus subtilis subsp.

Culture medium	Tryptone: 17.0, soy peptone: 3.0, sodium chloride: 5.0, dipotassium hydrogen phosphate: 2.5, glucose: 2.5g,pH:7.3±0.2(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	30 ℃;18-24h; aerobic;
Storage conditions	-20 ℃
Safety level	1
morphology	The colony is 1-2mm in diameter, round, with neat edges, opaque, gray-white on the front, gray-white on the back, convex in the middle, bright on the surface, smooth on the surface, moist in texture, easy to provoke, G + (blue-purple), bacilli
application	research; analysis and detection. 2015 Edition of Chinese Pharmacopoeia Quality Control Strains
Sharing mode	Public welfare sharing