BeNa Culture Collection
Culture medium | Bovine brain extract powder: 4.0, bovine heart extract powder: 4.0, peptone: 5.0, casein peptone: 16.0, sodium chloride: 5.0, glucose: 2.0, disodium hydrogen phosphate: 2.5, agar: 13.5,pH:7.4±0.2(25 ℃) |
Subculture procedure | Brain extract powder: 5.0g, heart extract powder: 12.5g, peptone: 5.0g, tryptone: 10.0g, sodium chloride: 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 15.0g (not included in liquid medium),pH 7.4±0.2. 121 ℃,15min sterilization. |
Growth conditions | 30°C;18-24h; aerobic |
Storage conditions | -20 ℃ |
Safety level | 1 |
morphology | The colony is 1-2mm in diameter, round, with neat edges, opaque, gray-white on the front, gray-white on the back, convex in the middle, bright on the surface, smooth on the surface, moist in texture, easy to provoke, G + (blue-purple), bacilli, purity: pure |
application | research; analysis and detection. 2015 Edition of Chinese Pharmacopoeia Quality Control Strains |
Sharing mode | Public welfare sharing |