enteritis salmonella typhimurium quantitative strain|BNCC353736 |BNCC

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Quantitative strains of Salmonella enterica subsp. enterica serovar Typhimurium

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	37 ℃;18-24h; aerobic
Storage conditions	-20 ℃
Safety level	2
morphology	The colony is 1-2mm in diameter, round, with neat edges, opaque, gray-white on the front and gray-white on the back, flat in shape, smooth on the surface, bright on the surface, moist in texture, easy to provoke, G-(red), bacilli, purity: pure
Separation substrate	Tissue, animal - pools of heart and liver from 4-week-old chickens
application	Control Culture Media Testing Prep Testing Control Intestinal Research Emerging Infectious Disease Research Pharmaceutical and Personal Care Water Testing
Sharing mode	Public welfare sharing