Escherichia coli negative quality control sample (multi-tube fermentation method)|BNCC385233 |BNCC

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Escherichia coli negative quality control sample (multi-tube fermentation method)

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  • number：BNCC385233
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Escherichia coli negative quality control sample (multi-tube fermentation method)
Culture medium Bovine brain extract powder: 4.0, bovine heart extract powder: 4.0, peptone: 5.0, casein peptone: 16.0, sodium chloride: 5.0, glucose: 2.0, disodium hydrogen phosphate: 2.5, agar: 13.5,pH:7.4±0.2(25 ℃)
Subculture procedure Brain extract powder: 5.0g, heart extract powder: 12.5g, peptone: 5.0g, tryptone: 10.0g, sodium chloride: 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 15.0g (not included in liquid medium),pH 7.4±0.2. 121 ℃,15min sterilization.
Growth conditions 37 ℃,18-24h, aerobic
Storage conditions -20 ℃
Safety level 2
Sharing mode Public welfare sharing
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