BeNa Culture Collection
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| Culture medium | Special peptone: 23.0, soluble starch: 1.0, sodium chloride: 5.0, agar: 10.0, defibrated sheep blood: 5% (added at 50 ℃),pH:7.3±0.2(25 ℃) |
| Subculture procedure | ① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried; |
| Growth conditions | 37 ℃;48-72h; anaerobic; |
| Storage conditions | -20 ℃ |
| Safety level | 1 |
| Sharing mode | Public welfare sharing |
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