BeNa Culture Collection

Mouse melanoma cells-BNCC
  • Mouse melanoma cells-BNCC
  • Mouse melanoma cells-BNCC
【B16-F1】

Mouse melanoma cells

  • Price: $416
  • number:BNCC250440
  • Form:
    Fibroblast-like, long spindle-shaped, with neat edges, monolayer growth, pigment production, no vacuoles, black spots in cell secretions
Basic package DNA extraction
Package:
  • Package A:mycoplasma detection assay + 2 vials of frozen cell
  • Package B:mycoplasma detection assay + 2 vials of frozen cell + 100ml of complete medium
Essential Information Related Products
Mouse melanoma cells
Culture medium RPMI-1640 complete medium: 90% RPMI-1640 + 10% FBS
Subculture procedure Recovery steps: ① The frozen vial is taken out of liquid nitrogen or -80 ℃ refrigerator and put into PE gloves, quickly submersed into a 37 ℃ water bath pan, shaken The frozen vial to accelerate dissolution, and it is advisable to dissolve all within 1min; (2) Add the dissolved cell solution into a centrifuge tube containing 9ml of complete culture medium in an ultra-clean table, centrifuge at 1000-1200rpm for 5min, discard the supernatant, resuspend the cells with 1-2ml of complete media. (3) The cell suspension was added to T25 flask containing 5-6mL complete medium and cultured in an incubator.
cell subculture: ① remove the medium, rinse twice with PBS, and add 1-2mL pancreatin (0.25% Trypsin + 0.02% EDTA); (2) Observe the digestion situation under the microscope. When the cell edge shrinks and the adherent is loose (a pipette can be used to suck up some pancreatin and gently blow somewhere in the cell layer, and the cell layer can be seen to detach with naked eyes, I .e. digestion is completed, otherwise digestion is continued). directly suck out pancreatin, add 5-6mL of complete medium, gently blow the cell layer off. (3) dispense the cell suspension into a fresh T25 flask as a ratio of 1:2, add appropriate complete culture medium, mix the cell suspension evenly, and culture in an incubator.; ④ Pay attention to the change of pH of medium and cell density, renew the medium regularly (2-3 times a week), and repeat the subculture or cryopreservation when the cell density reaches 80%-90%.
Growth conditions 37 ℃;5% CO2 + 95% air;
Growth characteristics Adherent growth
Storage conditions Liquid nitrogen
Safety level 1
morphology Fibroblast-like, long spindle-shaped, with neat edges, monolayer growth, pigment production, no vacuoles, black spots in cell secretions
Sharing mode Public welfare sharing
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