BeNa Culture Collection

Human megakaryocytic leukemia cells-BNCC
Human megakaryocytic leukemia cells-BNCC
  • Human megakaryocytic leukemia cells-BNCC
  • Human megakaryocytic leukemia cells-BNCC
  • Human megakaryocytic leukemia cells-BNCC
【MEG-01】

Human megakaryocytic leukemia cells

  • Price: $273
  • number:BNCC359441
  • Form:
    Lymphoblasts, round
Basic package DNA extraction
Package:
  • Package A:STR report + 2 vials of frozen cell
  • Package B:STR report + 2 vials of frozen cell + 100ml of complete medium
Essential Information Certificate Related Products
Human megakaryocytic leukemia cells
Culture medium RPMI-1640 complete medium: 90% RPMI-1640 + 10% FBS
Subculture procedure Recovery steps: ① Take out the frozen vial from liquid nitrogen or -80 ℃ and put it into PE gloves, quickly submerse into a 37 ℃ water bath pot, shake the frozen vial to accelerate dissolution, and it is advisable to dissolve all within 1 minute; (2) Add the dissolved cell liquid into a centrifuge tube containing 9ml of complete culture medium in an ultra-clean table, centrifuge at 1000-1200rpm/min for 5 minutes, discard the supernatant, resuspend the cells with 1-2ml of complete media. ③The cell suspension was then added to a T25 flask containing 6-8mL complete medium and placed in an incubator for upright cultivation. Cell subculturing: ① Centrifugation: collect cells, centrifuge at 1000rpm for 5 minutes, discard the supernatant, add 1-2mL of culture solution and blow well, and dispense the cell suspension into a fresh T25 flask containing 8ml of culture medium according to a ratio of 1:2. (2) Half-volume liquid medium exchange: after the culture flask is allowed to stand for 5-10 minutes, half of the supernatant medium is gently sucked away, the remaining medium with cell precipitation is resuspended and mixed evenly, and the cell suspension is divided into a fresh T25 flask containing 8ml of medium according to a ratio of 1:2. (3) Pay attention to the change of pH of medium and cell density, renew the medium regularly (2-3 times a week), and repeat the subculture or cryopreservation when the cell density reaches more than 2 × 106 cells/ml.
Growth conditions Culture temperature 37 ℃; Atmosphere 5% CO2 + 95% air;
Growth characteristics Suspension growth
Storage conditions Liquid nitrogen
Type Megakaryocytic leukemia cells
Safety level 1
morphology Lymphoblasts, round
Separation substrate Bone; Bone marrow megakaryocyte mother cell; Chronic myeloid leukemia (CML)
Sharing mode Public welfare sharing

Human megakaryocytic leukemia cells MEG-01

BNCC number: 359441

Growth characteristics: suspension growth

Growth conditions: 37 ℃,5% CO2

Complete medium: 90% ECM + 10% FBS

Cryopreservation conditions: 50%RPMI-1640+40%FBS+10%DMSO

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the cells are well. For the frozen cells,  it shall be stored in the refrigerator at -80 ℃ upon arrival. If they are not used for a long time, they shall be transferred to liquid nitrogen for storage overnight.  Recovered cells in T25 culture flask, upon receipt, put the culture flask in the incubator for 2-3h, and then carry out handling procedure. Under aseptic conditions, transfer the original flask culture solution to a centrifuge tube, centrifuge at 1000-1200rpm/min for 5-10 min, resuspend the cells, transfer the suspended cells to a new T25 flask, add complete culture medium to 10ml, and put it into an incubator for culture. Please strictly follow this instruction, otherwise no reissue service will be provided if cell inactivation is caused.

Recovery steps: 
(1)The frozen vial is taken out of liquid nitrogen or -80 ℃ refrigerator and put into PE gloves, quickly submersed into a 37 ℃ water bath, shaken the frozen vial to accelerate dissolution, and it is advisable to dissolve all within 1min; 
(2)Put the dissolved cell solution into a centrifuge tube containing 9ml of complete culture medium in an ultra-clean table, centrifuge at 1000-1200rpm for 5min, discard the supernatant,  resuspend the cells with 1-2ml of complete media. 
(3)The cell suspension was added to T25 flask containing 6-8mL complete medium and put it into an incubator for erection culture.

Cell subculturing: 
① Centrifugation: collect cells, centrifuge at 1000rpm for 5 minutes, discard the supernatant, add 1-2mL of culture solution and blow well, and dispense the cell suspension into a fresh T25 flask containing 8ml of culture medium according to a ratio of 1:2. 

② Half-volume solution exchange:  the culture flask is sit for 5-10 minutes, half of the supernatant medium is gently sucked away, the remaining medium with cell precipitation is resuspended and mixed evenly, and the cell suspension is dispensed into a fresh T25 flask containing 8ml of medium according to a ratio of 1:2. 

③ Pay attention to the change of pH value of media and cell density, renew the media regularly (2-3 times a week), and repeat the subculture or cryopreservation when the cell density reaches more than 2 × 10⁶ cells/ml.

Notes:
(1)The cells are density dependent. Initiate the subcultivation in T25 flask when the cell density reaches 80%; A subcultivation ratio of 1:2 is recommended. 
(2)If the cells in sealed culture bottle, put it into the incubator for cultivation after handling, with the cap unscrewed.

Recovery record:  According to the recovery instructions, the results of the cell recovery are reported as follows:

Item quality standard Recovery record
viability: suspension growth rate ≥ 80.0% in 148 hours adherence is observed in 18 hours, cell adherence rate ≥ 80.0% in 132 hours
cell morphology: suspension, lymphoblast suspension, lymphoblast, round
attached:
conclusion: good viability, no abnormal cell morphology, qualified;

 

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