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Jurkat human acute T lymphocyte leukemia cells
suspension, lymphoblast-like
No. : 338495
culture :37 ℃,5% CO2 CM12-1 liquid medium
product format : 2ml frozen vial x 2, or 15mL centrifuge tube
Biosafety level : 1, handle in ultra-clean table or safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the cells are well. For the frozen cells, it shall be stored in the refrigerator at -80 ℃ upon arrival. If they are not used for a long time, they shall be transferred to liquid nitrogen for storage overnight. Cells in centrifuge tube, upon receipt, put the culture flask in the incubator for 4h, and then carry out handling procedure. During recovery, each vial shall be used up once and shall not be retained. After recovery, the cells can be passed on to the next generation and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of cells are not be available in case of loss of cell viability.
culture conditions :37 ℃,5% CO2,CM12-1 culture solution. CM12-1 culture solution: 85% RPMI-1640 + 15% FBS. RPMI-1640:1640 culture solution, containing glutamine.
Recovery steps:
(1)prepare a new 100mm culture dish containing 12ml of the above culture medium;
(2)remove the frozen vial from liquid nitrogen or refrigerator at -80℃, thaw the vial in 37°C water bath for 1-2 minutes. Transfer the vial to biosafety cabinet for culture as soon as the contents are completely thawed.
(3)draw the solution with a sterile pipette, drip into a new culture dish, and mix it evenly by shaking clockwise;
(4)Put it into incubator (37℃,5%CO2), change the media overnight, and it will grow up in 5-7 days.
Subculture: the suspended cells were gently blowed and evenly distributed into 3-6 fresh culture medium dishes. After being shaken well, the cells were put into the incubator for culture
Cryopreservation: The suspended cells were transferred to the centrifuge tube for (110g, 3min) centrifugation,after centrifugationterminate digestion with 3ml of cryopreservation solution (90%FBS+10%DMSO), aspirate and dispense into 3 frozen vials, and cryopreserve at -80 ℃ with a programmed cooling box.
Recovery record: According to the recovery instructions, the results of the cell recovery are reported as follows:
suspension growth rate ≥ 80.0% in 120 hours
inoculation with 20% cell solution, suspension growth rate reaches 40.0%
in 18 hours, and 80.0% in 108 hours